LOCUS       sequence-129083-         667 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression of CENP-B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 667)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG141
FEATURES             Location/Qualifiers
     source          1..667
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..64
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             77..121
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 cggaggggat cggtggagct ccaccggtgg cggccgctct agagagaatc tttattttca
       61 gggcgccgcc aaattcaaag aaaccgctgc tgctaaattc gaacgccagc acatggacag
      121 cggaggtgga ggtactagtc tcgacggccc caagaggcga cagctgacgt tccgggagaa
      181 gtcacggatc atccaggagg tggaggagaa tccggacctg cgcaagggcg agatcgcgcg
      241 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      301 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      361 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      421 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcnc atagccgagg agctgggcat
      481 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      541 gtcctgcagc ggcgtgnccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      601 gcctgccagt ccggccgcgg tgccctcgga nggcantggc gngagcacta ctggttggcg
      661 cgctnng
//
