LOCUS       sequence-129178-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TCR beta chain transgene expression plasmid which has a unique ClaI 
            site for the insertion of a TCR beta chain VDJ exon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Barnden MJ, Allison J, Heath WR, Carbone FR
  TITLE     Defective TCR expression in transgenic mice constructed using 
            cDNA-based alpha- and beta-chain genes under the control of 
            heterologous regulatory elements.
  JOURNAL   Immunol Cell Biol. 1998 Feb;76(1):34-40.
  PUBMED    9553774
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol."; date: "1998-02"; volume: "76(1)"; pages: "34-40"
COMMENT     Sequence Label: p3A9cbetaTCR
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatct gcatggtgca tactgaaggc ttctgtttcc tgtgtgactt ctaacagatg
      121 taagatagtt tagatggggg gcttgagatg agatatatgg gttccccttt agagaccctc
      181 ctcttggttg ttctcactgt cacggtgata gctagaggct gaggtagaaa gggctgcatg
      241 agaagggttt gaagacagga tgtggcaagt gtggttccca aaatgctcag aatgtgagtg
      301 agtgggggag cattcacagg caccccagat gtaggacctg gtaaatgtca aaccacatcc
      361 tgttgtgact cctggagatg gagctatggc aggtggccat tcttacttgg tgctgatgtt
      421 acttacagat aaacagccag acctggggtg agatgcttcc accccttccc ccattgtctt
      481 caggaaccga aaagtcccag gaatgcatct cttcacttgc tgatgtttga tgaaaaacag
      541 ttcttcttaa gattattttt tttatcatgt tcctatatct ctttgctgga atcggtcagg
      601 gaaacaagta ttaagggtcc tactgtgcct ggttaactat gggactcaag atcagcactg
      661 tccctttgca ggtcagatgc cttgtaagat atatcacaat gtgtgtgtct ggagctggga
      721 aggacttaga tttccccact taccattctg tcagtgctac aaggcctgca gaattttcat
      781 caacaaacca ctaagta
//
