LOCUS       sequence-129187-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human full length SENP7. Suitable for in vitro 
            transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP7
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..94
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca
       61 tcatcacagc agcggcctgg tgccgcgcgg cagccatatg gtacctatgg acaagagaaa
      121 gctcgggcga cggccatctt catccgaaat catcacagaa ggaaaaagga aaaagtcatc
      181 ttctgattta tcggagataa gaaagatgtt aaatgcaaaa ccagaggatg tccatgttca
      241 atcaccactg tccaaattca gaagctcaga acgctggact ctccctttgc agtgggaaag
      301 aagcctaagg aataaagtca tctctctaga ccataaaaat caaaaacata tccgagggtg
      361 tcctgttact tccaagtcat caccagaaag gcaactcaaa gttatgttga cgaatgtcct
      421 atggacggat ttaggacgaa aattcagaaa gaccctacct agaaacgatg ctaatttatg
      481 tgatgccaac aaggtgcaat cagactcatt gccttcgaca tctgttgaca gcctagagac
      541 atgtcaaaaa ttagaacctc ttcgccaaag ccttaattta tctgaaagga tacccagagt
      601 tatattgacg aatgtcctgg gaacggagtt aggaagaaaa tacataagga ccccacctgt
      661 aactgaggga agtttgagtg atacagacaa cttgcaatca gagcaacttt cttcatcatc
      721 tgatggcagc ctagaatctt atcaaaatct aaaccctcac aagagctgtt atttatctga
      781 aaggggctca caacgaagta agacagtaga tgacaattct gcaaagcaga ctgcgcacaa
      841 taaagaaaaa cgaagaaagg atgatggcat ttctctttta atatctgata ctcagcctga
      901 agaccttaac agtggaagta gaggttgtga tcatctcgaa caggaaagca gaaacaagga
      961 tgt
//
