LOCUS       sequence-129212-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Alpha plant expression vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_alpha2
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    147..171
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    250..271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(286..291,292..309,310..316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    324..340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     329..351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     348..364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     348..364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    377..433
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(434..451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(434..450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(443..465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(659..678)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    800..822
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgagat
      121 ctcaaacaaa cacatacagc gacttagttt acccgccaat atatcctgtc aagcttcgtc
      181 tcaagcgaga gaccccagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actatatgct
      301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      361 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
      421 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      481 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      541 cctgcgccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      601 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      661 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      721 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg ggtctctctc
      781 ctgactgaga cgaagcttgt tacaccacaa tatatcctgc caagatctaa ttccggg
//
