LOCUS       sequence-129252-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(SnaBI)
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             32..259
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             260..487
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             488..715
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             716..943
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcagatc ttcgtgaaga ccctgaccgg
       61 caagaccatc accctagagg tggagcccag tgacaccatc gagaacgtga aggccaagat
      121 ccaggataaa gagggcatcc cccctgacca gcagaggctg atctttgccg gcaagcagct
      181 ggaagatggc cgcaccctct ctgattacaa catccagaaa gagtcaaccc tgcacctggt
      241 cctccgtctg aggggtggca tgcagatctt cgtgaagacc ctgaccggca agaccatcac
      301 cctggaggtg gagcccagtg acaccatcga gaatgtgaag gccaagatcc aggataaaga
      361 gggcatcccc cctgaccagc agaggctgat ctttgccggc aagcagctgg aagatggccg
      421 caccctctct gattacaaca tccagaagga gtcaaccctg cacctggtcc tccgtctgag
      481 gggtggcatg cagatcttcg tgaagaccct gactggcaag accatcaccc tggaggtgga
      541 gcccagtgac accatcgaga acgtgaaggc caagatccag gataaagagg gcatcccccc
      601 tgaccagcag aggctgatct ttgccggcaa gcagctggaa gatggccgca ccctctctga
      661 ttacaacatc cagaaggagt caaccctgca cctggtcctt cgcctgagag gtggcatgca
      721 gatcttcgtg aagaccctga ccggcaagac catcaccctg gaggtggagc ccagtgacac
      781 catcgagaat gtgaaggcca agatccagga taaagagggc atcccccctg accagcagag
      841 gctgatcttt gccggcaagc agctggaaga tggccgcact ctctctgatt acaacatcca
      901 gaaagagtcg accctgcacc tggtcctccg tctgaggggt ggctacgtat tattcggtct
      961 gcggatccga attcgagctc cg
//
