LOCUS       sequence-129349-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  polycistronic cloning vector for pST44 plasmid suite.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST44
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(60..77)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(60..76)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(115..133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             370..561
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(555..577)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cgctggtacc gatatcgagc tcgactggga aaaccctggc gaagcttccc ggggaattca
       61 ctggccgtcg ttttacaaga tctaggcctt ctagagggaa accgttgtgg tctccctata
      121 gtgagtcgta ttaatttcgc gggatcgatg catggtgcca acacctacat ctgtattaac
      181 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag
      241 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
      301 gagcatcctc tctcgtttca tcggtatcat taccccatga acagaaatcc cccttacacg
      361 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
      421 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
      481 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
      541 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
      601 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      661 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
      721 catcagagca ga
//
