LOCUS       sequence-129366-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 4 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc4-DHFRCYD
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             549..571
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(608..627)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(609..627)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gttagcagcc ggatcagcgc gcacgcgttg tacattaagc gcaaaattcg gtgccggcac
       61 gacgctcgag gatttcgaaa caatagctat gcgagttctg cgcatcagca tcgtggaatt
      121 cgctgaatac cgattcccag tcatccggct cgtaatccgg aaaatgggtg tcgccttcca
      181 cttctgcatc gatatgcgtc agatacagtt tttgcgcttt tggcaagaac tgttcataaa
      241 cgcgtccgcc gccaatcacc atgatttctg gtacgtcacc acacgccgcg atggcttcgt
      301 cgaccgattt aacccaggta acccgatcat cggtcccggg ttgactgctg aggataatat
      361 ttttgcggcc gggcaaaggc ctaccgattg attcccaggt atggcgcccc ataatcacgg
      421 gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc catggcatgg
      481 cgttttccat gccgataacg cgatctaccg ctagcgccgc aatcagactg atggatccca
      541 tatgtatatc tccttcttaa agttaaacaa aattatttcc ggagccggag ggaaaccgtt
      601 gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gatgcatggt gccaacacct
      661 acatctgtat taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc
      721 atccataccg ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca
//
