LOCUS       sequence-129392-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_722_S2-1_CGCG-to-HEY
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(555..579)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(842..860)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ccagaacatt tctctatcga taggtaccac aagtttgtac aaaaaagcag gctccgaatt
       61 cgccctttgc acaatttctg tgaagtgaca gccactgaga aatagtgtca agtagctctc
      121 gtccgctaat atcaatttag acgatataca tacggccaga tctggcacgc agtctgcatt
      181 cccaatgatg cgggatggat ttggattgtg gggcggaatc tctaagtgcg ataagatgcg
      241 gagaattagg ggcacgtgtc ggctgtggct ccgttttgtt aaatatacta tgtgtgtatg
      301 tgcgtagtac agcgacttgt acggagtaca taaacatatg catatctgcg gtttagttgt
      361 cgacattatc ttttgttcgt cgtttgaaaa atgtgtgtag ttgttggggt aggggcacgt
      421 gtcggctgat tgccgggcta cagggtgctt tgagtctttg tttttggatt tcgttttcga
      481 tgtttagcta cgaaagccaa gcgttgtgct attttcgaat tttgtatttt ataacccggg
      541 caagggcgaa ttcgacccag ctttcttgta caaagtggta gatctgcgat cgagctcgcc
      601 cggggatcga gcgcagcggt ataaaagggc gcggggtggc tgagagcatc agttgtgaat
      661 gaatgttcga gccgagcaga cgtgccgctg ccttcgttaa tatcctttga ataagccaac
      721 tttgaatcac aagacgcata ccaaacggca ttccggtact gttggtaaag ccaccatgga
      781 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      841 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      901 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      961 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1021 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1081 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     1141 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa att
//
