LOCUS       sequence-129413-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-His tagged nonglycosylated human serum transferrin 
            unable to release iron from the N-lobe or the C-lobe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Halbrooks PJ, He QY, Briggs SK, Everse SJ, Smith VC, MacGillivray 
            RT, Mason AB
  TITLE     Investigation of the mechanism of iron release from the C-lobe of 
            human serum transferrin: mutational analysis of the role of a pH 
            sensitive triad.
  JOURNAL   Biochemistry. 2003 Apr 8;42(13):3701-7.
  PUBMED    12667060
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2003-04-8"; volume: "42(13)"; pages: 
            "3701-7"
COMMENT     Sequence Label: pNUT N6His K206E/K534A hTFNG
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccaccccta gagtcgactc aatcaatcag cggccgccct cgagctatta aggtctacgg
       61 aaagtgcagg cttccaggag tgatgaggtg gagcattttc tcaggttacc aacagccttg
      121 acatattctt ctcctaagta tttttcatat gtgtttctgt catgaagttt ggccaaacat
      181 actgtgtcat ctctgaacag aaggtccttg gtttccgacc ggaacaaaca aaagttgccc
      241 gagcagtcag ttacgtcgct tccaaatagg tgctgctgtt gacgtaatat cttgtggacg
      301 caagcttcct tatctttccg tgtgaccaca gcgtgattcg gggctctggc caggtggcag
      361 ttcgcatact cctccacagg tttcctggta ccatcaaggc acagcaactc atagtctttt
      421 tcattcagat tcttagccca tggatcaggg ttttttcccc cagtgttctg tgggacagtc
      481 tggtgtgcca caaaagccac atctcccttc tcaaccagac acctgaaagc gcctgtgtag
      541 ccgtagtatc cctctttgtt gttgggttca cacaggttta ggcctgagcc catacacagc
      601 ttacagagac tggagtcttt cttagaccca ggggcacaac cttcactgaa aaattcatca
      661 aatctgcagt ggttgatctt attgtagagc aggcccatgg ggatgttcca gccagcggtt
      721 ctgccaactg ccgtatggca ggacttcttg cctttcagat tgtcccaggt gaggtcagaa
      781 gctgatttct tcaccactgc tacagcaaaa taccctgcct ctggtgtatc ctcacaatta
      841 tcgctcttat cgtagtt
//
