LOCUS       sequence-129453-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd gen lentiviral vector. Cre-removable and tet/dox controllable 
            expression of FLAG-HA-tagged transgene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, Garzia
            A, Morozov P, Manickavel S, McFarland HL, Roeder RG, Hafner M, 
            Tuschl T
  TITLE     DND1 maintains germline stem cells via recruitment of the CCR4-NOT 
            complex to target mRNAs.
  JOURNAL   Nature. 2017 Mar 23;543(7646):568-572. doi: 10.1038/nature21690. 
            Epub 2017 Mar 15.
  PUBMED    28297718
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21690"; journalName: "Nature"; date: "2017-03-23- 
            23"; volume: "543"; issue: "7646"; pages: "568-572"
COMMENT     Sequence Label: pLVE-FH-IN
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             2..182
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    202..235
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(669..688)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
       61 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
      121 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      181 ccagtacaag caaaaagcag cataacttcg tataatgtat gctatacgaa gttattccca
      241 tggtgaattg atcccatctt gtcttcgttg ggagtgaatt agcccttcca gtcccccctt
      301 ttcttttaaa aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac
      361 caataactta aaactgccac acacaaaaaa aatggtccac aacattcttt ccttctgaag
      421 gttttacgat gcattgttat cattaaccag tcttttacta ttaaacttaa atggccaatt
      481 gagacaaaca gttctgagac cgttcttcca ccactgatta agactggggt ggcaggtgtt
      541 aggggtaata tgaattctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
      601 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
      661 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
      721 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
      781 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
      841 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
//
