LOCUS       sequence-129498-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 600 bp homology arms to SS9 for one-step markerless genome 
            integration in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: pSS9
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcttggttga gaatacgccg aagttaaaat cagcccctgg ctggcaacgg cgtcgatatc
       61 tggtgtaggg ttacccaccg ccacgccgcc accgttaaaa ccgacgtcca tccagcccac
      121 atcgtccagc aagaaaacaa ccacattcgg tttcttaccg gtttttttct caagttctgc
      181 cagcttctgc tgggtttctt tatcctgcgc cggatgctgc attactggca tcatattgtc
      241 ggcaatagtg gtcgccggtt taaccagata ctggtttggg tgatcgtatc cggcaaagcc
      301 tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg gccatgagag gaagagcggc
      361 ggcgacagca acaacaagac gtttgggtga aaacgaaaat tccatgcaaa atgctccggt
      421 ttcatgtcat caaaatgatg acgtaattaa gcattgataa ttgagatccc tctccctgac
      481 aggatgatta cataaataat agtgacaaaa ataaattatt tatttatcca gaaaatgaat
      541 tggaaaatca ggagagcgtt ttcaatccta cctctggcgc agttgatatg tcaaacaggt
//
