LOCUS       sequence-129499-         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 600 bp homology arms to SS9 for one-step markerless genome 
            integration in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Bassalo MC, Garst AD, Halweg-Edwards AL, Grau WC, Domaille DW, 
            Mutalik VK, Arkin AP, Gill RT
  TITLE     Rapid and Efficient One-Step Metabolic Pathway Integration in E. 
            coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 22.
  PUBMED    27072506
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 22."
COMMENT     Sequence Label: pSS9
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttattatatc gcgttgatta ttgatgctgt ttttagtttt aacggcaatt aatatatgtg
       61 ttattaattg aatgaatttt atcattcata ataagtatgt gtaggatcaa gctcaggtta
      121 aatattcact caggaagtta ttactcagga agcaaagagg attacagaat tatctcataa
      181 caagtgttaa gggatgttat ttcccgattc tctgtggcat aataaacgag tagatgctca
      241 ttccatctct tatgttcgcc ttagtgcctc ataaactccg gaatgacgca gagccgttta
      301 cggtgcttat cgtccactga cagatgtcgc ttatgcctca tcagacacca tggacacaac
      361 gttgagtgaa gcacccactt gttgtcatac agacctgttt taacgcctgc tccgtaataa
      421 gagcaggcgt ttttttatgt atcaggaagg ccccggaggt gcttgcctcc gggtgagaag
      481 gaactactgt ggcgggttat tctgcaacgt taacatcaaa ccgtcgcgac gcatagctgc
      541 agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc
//
