LOCUS       sequence-129738-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to produce AAV expressing the FlpO recombinase and pig gRNA 
            towards 3 tumor suppressors: PTEN, p53 and SMAD4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Berthelsen MF, Riedel M, Cai H, Skaarup SH, Alstrup AKO, 
            Dagnaes-Hansen F, Luo Y, Jensen UB, Hager H, Liu Y, Callesen H, 
            Vendelbo MH, Jakobsen JE, Thomsen MK
  TITLE     The CRISPR/Cas9 Minipig-A Transgenic Minipig to Produce Specific 
            Mutations in Designated Tissues.
  JOURNAL   Cancers (Basel). 2021 Jun 16;13(12). pii: cancers13123024. doi: 
            10.3390/cancers13123024.
  PUBMED    34208747
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2021-06-16"; volume: "13(12). pii: 
            cancers13123024. doi"; pages: " 10.3390/cancers13123024"
COMMENT     Sequence Label: AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaggaagg tgatgagcca gttcgacatc ctgtgcaaga ccccccccaa ggtgctggtg
       61 cggcagttcg tggagagatt cgagaggccc agcggcgaga agatcgccag ctgtgccgcc
      121 gagctgacct acctgtgctg gatgatcacc cacaacggca ccgccatcaa gagggccacc
      181 ttcatgagct acaacaccat catcagcaac agcctgagct tcgacatcgt gaacaagagc
      241 ctgcagttca agtacaagac ccagaaggcc accatcctgg aggccagcct gaagaagctg
      301 atccccgcct gggagttcac catcatccct tacaacggcc agaagcacca gagcgacatc
      361 accgacatcg tgtccagcct gcagctgcag ttcgagagca gcgaggaggc cgacaagggc
      421 aacagccaca gcaagaagat gctgaaggcc ctgctgtccg agggcgagag catctgggag
      481 atcaccgaga agatcctgaa cagcttcgag tacaccagca ggttcaccaa gaccaagacc
      541 ctgtaccagt tcctgttcct ggccacattc atcaactgcg gcaggttcag cgacatcaag
      601 aacgtggacc ccaagagctt c
//
