LOCUS       sequence-129770-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knock down expression of spliced Copia in Drosophila melanogaster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  TITLE     Nilson lab plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCoHygro L16_shRNA of spliced Copia
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(128..407)
                     /label=copia promoter
                     /note="strong promoter from the Drosophila transposable 
                     element copia (Sinclair et al., 1986)"
     promoter        486..886
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
     primer_bind     complement(951..968)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(951..967)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(960..982)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtcagct caggctttcc catggtggcc aagctgggga
      121 tccccataac ctgttgtaat ttataattta tatttccttt cttaataaat aaataaatag
      181 tcaagtttat gtttgagttt tatgatttat attttaagtt atttcaactg caacaccagc
      241 accacgacct acttacagca aaaaacgtac aagaaggaaa gaaggaataa aaagagtggt
      301 attctcttac aatatgtttt atggcataaa aggtgtggcc attcatatca aatataaagt
      361 agtgttgttt aacgttactt ttgtaggttg aatagtatat tccaacagat gatgaggggt
      421 tcccaatcct aaacccattt gccgttccca gaagcatgaa accaccacgc accggatcct
      481 ctagagttcg actttgcagc ctgaaatacg gcacgagtag gaaaagccga gtcaaatgcc
      541 gaatgcagag tctcattaca gcacaatcaa ctcaagaaaa actcgacact tttttaccat
      601 ttgcacttaa atcctttttt attcgttatg tatacttttt ttggtcccta accaaaacaa
      661 aaccaaactc tcttagtcgt gcctctatat ttaaaactat caatttatta tagtcaataa
      721 atcgaactgt gttttcaaca aacgaacaat aggacacttt gattctaaag gaaattttga
      781 aaatcttaag cagagggttc ttaagaccat ttgccaattc ttataattct caactgctct
      841 ttcctgatgt tgatcattta tataggtatg ttttcctcaa tacttcgaaa tttagagaat
      901 taattgggat aaataccagt tgattctctg aatttctttt taagcttggc actggccgtc
      961 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg cctt
//
