LOCUS       sequence-129799-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing the GUS enzyme. Can be used to construct 
            transcriptional reporters. For use in plants and compatible with the
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p221a-GUS
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..57
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     41..57
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    98..197
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt caggaaacag ctatgaccat
       61 gtaatacgac tcactatagg ggatatcagc tggatggcaa ataatgattt tattttgact
      121 gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt
      181 gtacaagaaa gctgggtgtc attgtttgcc tccctgctgc ggtttttcac cgaagttcat
      241 gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt cgcgagtgaa
      301 gatccctttc ttgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa
      361 attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt gatacatatc
      421 cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt gcagcccggc
      481 taacgtatcc acgccgtatt cggtgatgat aatcggctga tgcagtttct cctgccaggc
      541 cagaagttct ttttccagta ccttctctgc cgtttccaaa tcgccgcttt ggacatacca
      601 tccgtaataa cggttcaggc acagcacatc aaagagatcg ctgatggtat cggtgtgagc
      661 gtcgcagaac attacattga cgcaggtgat cggacgcgtc gggtcgagtt tacgcgttgc
      721 ttccgccagt ggcgcgaaat attcccgtgc accttgcgga cgggtatccg gttcgttggc
      781 aatactccac atcaccacgc ttgg
//
