LOCUS       sequence-129807-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing Venus. Can be fused with linker to the 
            C-terminal end of protein of interest. For use in plants and 
            compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-VenusYFP-3AT
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..59
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    120..215
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(753..774)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catatggtcg acctgcaggc ggccgcgaat tcactagtga ttcaggaaac agctatgacc
       61 atgtaatacg actcactata ggggatatcg cggccgcgtt aacgctacca tggagctcca
      121 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
      181 cttttttata atgccaactt tgtataataa agttgccctc gacacaaaaa gcctatactg
      241 tacttaactt gattgcataa ttacttgatc atagactcat agtaaacttg attacacaga
      301 taagtgaaga aacaaaccaa ttcaagacat aaccaaagag aggtgaaaga ctgttttata
      361 tgtctaacat tgcaccttaa tatcacactg ttagttcctt tcttacttaa attcaaccca
      421 ttaaagtaaa aacaacagat aataataatt tgagaatgaa caaaaggacc atatcattta
      481 ttaactctta tccatccatt tgcattttga tgtccgaaaa caaaaactga aagaacacag
      541 taaattacaa gcagaacaaa tgatagaaga aaacagcttt tccaatgcca taatactcaa
      601 acttagtagg attctggtgt gtgggcaatg aaactgatgc attgaacttg acgaacgttg
      661 tcgaaaccga tgatacggac gaaagctggg aggcctggat cgactagtta cttgtacagc
      721 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      781 cgcttctcgt tggg
//
