LOCUS       sequence-129824-         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB410
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(77..201)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             212..277
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      296..548
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
       61 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      121 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      181 agctttcttg tacaaagtgg ttgataacag cgactacaaa gaccatgacg gtgattataa
      241 agatcatgac atcgactaca aggatgacga tgacaagtag agctcgaatt tccccgatcg
      301 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
      361 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
      421 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
      481 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
      541 actagatcgg gaattggttc cggaaccaat tcgtaatcat gtcatag
//
