LOCUS       sequence-129826-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing 3AT. Necessary to complete the 
            transcriptional reporters by providing the poly-A tail. For use in 
            plants and compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-3AT
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(154..277)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tatagggcga attgggcccg acgtcgcatg ctcccggccg ccatggcggc cgcgggaatt
       61 cgatttgtaa aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc
      121 tacaggtcac taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcaac tttcttgtac aaagtggaat aacaggcctc ccagctttcg
      301 tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcccaca
      361 caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt cttctatcat
      421 ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa
      481 atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta
      541 tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat
      601 taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat
      661 tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat
      721 tatgcaatca agttaagtac agtataggct ttttgtgtcg agg
//
