LOCUS       sequence-129827-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing 3AT. Necessary to complete the 
            transcriptional reporters by providing the poly-A tail. For use in 
            plants and compatible with the MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p2R3a-3AT
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..59
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    120..215
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    710..734
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 catatggtcg acctgcaggc ggccgcgaat tcactagtga ttcaggaaac agctatgacc
       61 atgtaatacg actcactata ggggatatcg cggccgcgtt aacgctacca tggagctcca
      121 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
      181 cttttttata atgccaactt tgtataataa agttgtgttt aaaccctcga cacaaaaagc
      241 ctatactgta cttaacttga ttgcataatt acttgatcat agactcatag taaacttgat
      301 tacacagata agtgaagaaa caaaccaatt caagacataa ccaaagagag gtgaaagact
      361 gttttatatg tctaacattg caccttaata tcacactgtt agttcctttc ttacttaaat
      421 tcaacccatt aaagtaaaaa caacagataa taataatttg agaatgaaca aaaggaccat
      481 atcatttatt aactcttatc catccatttg cattttgatg tccgaaaaca aaaactgaaa
      541 gaacacagta aattacaagc agaacaaatg atagaagaaa acagcttttc caatgccata
      601 atactcaaac ttagtaggat tctggtgtgt gggcaatgaa actgatgcat tgaacttgac
      661 gaacgttgtc gaaaccgatg atacggacga aagctgggag gcctgttatt ccactttgta
      721 caagaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      781 gcataaa
//
