LOCUS       sequence-12985-e        1953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Hari SB, Merritt EA, Maly DJ
  TITLE     Sequence determinants of a specific inactive protein kinase 
            conformation.
  JOURNAL   Chem Biol. 2013 Jun 20;20(6):806-15. doi: 
            10.1016/j.chembiol.2013.05.005.
  PUBMED    23790491
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.05"; journalName: "Chem Biol"; date: 
            "2013-06-20- 20"; volume: "20"; issue: "6"; pages: "806-15"
COMMENT     Sequence Label: Map3k1
FEATURES             Location/Qualifiers
     source          1..1953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..44
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc
       61 aaaatcggat gattacccag atctgggtac cgagaacctg tacttccaat ccaatgatgg
      121 gcatcaggac agcttacagg cgctggcccc cgccagctgt ctagaaaaca gctcccttga
      181 gcacacagtc catagagaga aaactggaaa aggactaagt gctacgagac tgagtgccag
      241 ctcggaggac atttctgaca gactggccgg cgtctctgta ggacttccca gctcaacaac
      301 aacagaacaa ccaaagccag cggttcaaac aaaaggcaga ccccacagtc agtgtttgaa
      361 ctcctcccct ttgtctcatg ctcaattaat gttcccagca ccatcagccc cttgttcctc
      421 tgccccgtct gtcccagata tttctaagca cagaccccag gcatttgttc cctgcaaaat
      481 accttccgca tctcctcaga cacagcgcaa gttctctcta caattccaga ggaactgctc
      541 tgaacaccga gactcagacc agctctcccc agtcttcact cagtcaagac ccccaccctc
      601 cagtaacata cacaggccaa agccatcccg acccgttccg ggcagtacaa gcaaactagg
      661 ggacgccaca aaaagtagca tgacacttga tctgggcagt gcttccaggt gtgacgacag
      721 ctttggcggc ggcggcaaca gtggcaacgc cgtcataccc agcgacgaga cagtgttcac
      781 gccggtggag gacaagtgca ggttagatgt gaacaccgag ctcaactcca gcatcgagga
      841 ccttcttgaa gcatccatgc cttcaagtga cacgacagtc actttcaagt ccgaagtcna
      901 tgaagntgag agaagtagct ttancgatcg ccatgngant cagcgtctca gatgccctcc
      961 ccatcgtcct cagntgcagt ggaaaatgga gaagatatta tcatcattca gcaggacaca
     1021 ccagaaactn tnaggacata ccaaagcgaa acagccttac agagaagacg ntgagtggct
     1081 gaaaggccag cagatagncc tcggagcatt ttcttcctgt taccaagcac aggatgtggg
     1141 gactgggact ttaatggctg tgaaacaggt gacgtacgtc agaaacacat cntccgagca
     1201 ggaggaggtg gtggaagcgt tgagggaaga gatccggatg atgggtcacc tcaaccatcc
     1261 aaacatcatc cggatgctgg gggccacgtg cgagaagagc aactacaacc tcttcattga
     1321 gtggatggcg ggaggatctg tggctcacct cttgagtaaa tacggagctt tcaaggagtc
     1381 agtcgtcatt aactacactg agcagttact gcgtggcctt tcctatctcc acgagaacca
     1441 gatcattcac agagacgtca aaggtgccaa cctgctcatt gacagcaccg gtcagaggct
     1501 gagaattgca gactttggag ctgctgccag gttggcatca aaaggaaccg gtgcaggaga
     1561 gttccaggga cagttactgg ggacaattgc attcatggcg cctgaggtcc taagaggtca
     1621 gcagtatggt aggagctgtg atgtatggag tgttggctgc gccattatag aaatggcttg
     1681 tgcaaaacca ccttggaatg cagaaaaaca ctccaatcat ctcgccttga tatttaagat
     1741 tgctagcgca actactgcac cgtccatccc gtcacacctg tccccgggtc tgcgcgacgt
     1801 ggcgctgcgc tgcttagaac ttcagcctca ggaccggcct ccgtccagag agctgctgaa
     1861 acatccggtc ttccgtacca cgtggtagca ttggaagtgg ataacggatc cgaattcgag
     1921 ctccgtcgac aagcttgcgg ccgcactcga gca
//
