LOCUS       sequence-129880-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene 
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Long S, Wang Q, Sibley LD
  TITLE     Analysis of non-canonical calcium dependent protein kinases in 
            Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9.
  JOURNAL   Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15
  PUBMED    26755159
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15"
COMMENT     Sequence Label: pLoxP-DHFR-mCherry
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(244..277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
       61 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      121 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      181 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagctg
      241 agcataactt cgtatagcat acattatacg aagttatcgg aaatacagaa gctgcccgtc
      301 tctcgttttc ctctcttttc ggagggatca gggagagtgc ctcgggtcgg agagagctga
      361 cgagggggtg ccagagaccc ctgtgtcctt tatcgaagaa aagggatgac tcttcatgtg
      421 gcatttcaca cagtctcacc tcgccttgtt ttctttttgt caatcagaac gaaagcgagt
      481 tgcgggtgac gcagatgtgc gtgtatccac tcgtgaatgc gttatcgttc tgtatgccgc
      541 tagagtgctg gactgttgct gtctgcccac gacagcagac aactttcctt ctatgcactt
      601 gcaggatggt gcagcgcaaa cgacggagag aaaggagcac cctctcagtt tccctacgat
      661 gtgctgtcag tttcgactct tcaccgcgaa cgattggcga tacgtctctg ttgacttgtt
      721 aggctccgac cacgaagctc ccttaactaa ataagccgcg acacctaagt gtacaccatt
      781 tgcagatcga taatctgcga ccgctgaatc cgtccagatc agtaaaaccg caccacc
//
