LOCUS       sequence-130089-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Kim KK, Yokota H, Santoso S, Lerner D, Kim R, Kim SH
  TITLE     Purification, crystallization, and preliminary X-ray 
            crystallographic data analysis of small heat shock protein homolog 
            from Methanococcus jannaschii, a hyperthermophile.
  JOURNAL   J Struct Biol. 1998 Jan . 121(1):76-80.
  PUBMED    9573624
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 1998 Jan . 121(1):76-80."
COMMENT     Sequence Label: pET21a.MJ0285
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             542..564
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    579..603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(603..622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(604..622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(706..725)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccctcgag tcattcaatg ttgattcctt tcttaattga
      121 ggattctgcc tttggtaata taactgataa aacaccattt tcaaacttag ctgaggcatt
      181 ttcttcctta acagttgcag gaagttttat tgttctatat atttcttcct cttctggaat
      241 ttctgagtag ataattcttt cactctcagt tatcattaat gggcttctct tagctctaat
      301 ctctaatgta tctccaactg catttaaaat tatgtcctct ttattaaccc ctggcaacca
      361 tgcaataact tttatatgct ggtctccttc aataattgag attggcatga acccttttcc
      421 agaaatttgt attcctgttg agctttgaat cattgtggtt cctgtcattg gtgttgcaaa
      481 aaactcttta aacattcttt caaataatga atcaaatggg tctcttccga acatatgtat
      541 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat
      601 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac
      661 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      721 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      781 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      841 tccttgcggc ggcggtgctc
//
