LOCUS       sequence-130169-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..459
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 atggcacacc atcaccacca tcacggatcc ttggctacta cacttgaacg tattgagaag
       61 aactttgtca ttactgaccc aagattgcca gataatccca ttatattcgc gtccgatagt
      121 ttcttgcagt tgacagaata tagccgtgaa gaaattttgg gaagaaactg caggtttcta
      181 caaggtcctg aaactgatcg cgcgacagtg agaaaaatta gagatgccat agataaccaa
      241 acagaggtca ctgttcagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc
      301 tttcacttgc agcctatgcg agatcagaag ggagatgtcc agtactttat tggggttcag
      361 ttggatggaa ctgagcatgt ccgagatgct gccgagagag agggagtcat gctgattaag
      421 aaaactgcag aaaatattga tgaggcggca aaagaactta tcaagtgtgt ggtggtggga
      481 gacggagctg taggtaaaac ttgcctactg atcagttaca caaccaatgc atttcctgga
      541 gaatatatcc ctactgtctt tgacaattat tctgccaatg ttatggtaga tggaaaaccg
      601 gtgaatctgg gcttatggga tacagctgga ctagaagatt atgacagatt acgcccccta
      661 tcctatccgc aaacagatgt gttcttaatt tgcttttccc ttgtgagtcc tgcatcattc
      721 caccacgtcc gtgcaaagtg gtatcctgag gtgcggcacc actgtcccaa cactcccatc
      781 atcctagtgg gaactaaact tgatcttagg gatgataaag acacgatcga gaaactgaag
      841 gagaagaagc tgactcccat cacctatccg cagggtctag ccatggctaa ggagattggt
      901 gctgtaaaat acctggagtg ctcggcgctc acacagcgag gcctcaagac agtgtttgac
      961 gaagcgatcc gagcagtcct ctgcccgcct cccgtgaaga agaggaagag aaaatgcctg
     1021 ctgttgtaaa agctt
//
