LOCUS       sequence-130183-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB211
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..198
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(239..544)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(341..360)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gtcatcgtca tccttgtagt cgatgtcatg atctttataa tcaccgtcat ggtctttgta
       61 gtcgctgtta tcaaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa
      121 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      181 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
      241 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      301 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
      361 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
      421 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
      481 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      541 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
      601 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacg
//
