LOCUS       sequence-130186-         602 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB220
FEATURES             Location/Qualifiers
     source          1..602
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(149..154,155..172,173..179)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(299..318)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
       61 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc
      121 tcgaggggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      181 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      241 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      301 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      361 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      421 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      481 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      541 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      601 tg
//
