LOCUS       sequence-130197-         712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB205
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(153..458)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(255..274)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tanattttgc
       61 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggtcgacct
      121 gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat caggttaatg
      181 gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc gataacggag
      241 accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc gatatgcacc
      301 accgggtaaa gttcacggga gactttatct gacagcagac gtgcactggc cagggggatc
      361 accatccgtc gcccgggcgt gtcaataata tcactctgta catccacaaa cagacgataa
      421 cggctctctc ttttataggt gtaaacctta aactgcattt caccagcccc tgttctcgtc
      481 agcaaaagag ccgttcattt caataaaccg ggcgacctca gccatccctt cctgattttc
      541 cgctttccag cgttcggcac gcagacgacg ggcttcattc tgcatggttg tgcttaccag
      601 accggagata ttgacatcat atatgccttg agcaactgat agctgtcgct gtcaactgtc
      661 actgtaatac gctgcttcat agcatacctc tttttgacat acttcgggta ta
//
