LOCUS       sequence-130255-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB307
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(148..153,154..171,172..178)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(298..317)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
       61 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct
      121 cgaggggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      181 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
      241 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      301 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      361 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      421 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      481 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      541 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      601 gtgg
//
