LOCUS       sequence-130265-         517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB411
FEATURES             Location/Qualifiers
     source          1..517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..176)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             187..252
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
       61 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
      181 aacagcgact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
      241 gacgatgaca agtagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
      301 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
      361 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
      421 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
      481 taggataaat tatcgcgcgc ggtgtcatct atgttac
//
