LOCUS       sequence-130351-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone containing AKT-PH with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-AKT-PH
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(583..682)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
       61 aactttgtac aaaaaagcag gctgccacca tgagcgacgt ggctattgtg aaggagggtt
      121 ggctgcacaa acgaggggag tacatcaaga cctggcggcc acgctacttc ctcctcaaga
      181 atgatggcac cttcattggc tacaaggagc ggccgcagga tgtggaccaa cgtgaggctc
      241 ccctcaacaa cttctctgtg gcgcagtgcc agctgatgaa gacggagcgg ccccggccca
      301 acaccttcat catccgctgc ctgcagtgga ccactgtcat cgaacgcacc ttccatgtgg
      361 agactcctga ggagcgggag gagtggacaa ccgccatcca gactgtggct gacggcctca
      421 agaagcagga ggaggaggag atggacttcc ggtcgggctc acccagtgac aactcagggg
      481 ctgaagagat ggaggtgtcc ctggccaagc ccaagcaccg cgtgaccatg aacgagtttg
      541 agtacctgaa gctgctgggc aagggcactt tcggcaaggt ggacccagct ttcttgtaca
      601 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
      661 gtcaaaataa aatcattatt tgccatcca
//
