LOCUS       sequence-130354-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of 
            p3E-ML1N*2(7Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: p3E-ML1N*2(7Q)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..160)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(630..725)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(762..781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(763..781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(786..802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(786..802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata tgttgtgttt
       61 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      121 cattttacgt ttctcgttca actttcttgt acaaagtggt gtattctgat ctgactatgg
      181 ctacacctgc cgggagacgc gcaagcgaaa ctgaaagact gctgacacca aaccctggct
      241 atggaaccca agtgggcaca tctccagctc ccaccacacc cactgaggaa gaggacctgc
      301 agcagcagct gaagtacttc tttatgagtc catgcgatca gttccaggca aaggggcagc
      361 agccctgtaa actgatgctg agaatcctga caatggccac tcccgctggc aggagagcaa
      421 gcgagactga aaggctgctg acccctaacc caggctacgg aacccaggtc ggaacatccc
      481 cagcacctac tacccctacc gaagaggaag acctgcaaca acagctgaaa tatttcttta
      541 tgagcccttg cgatcagttt caggccaagg ggcagcagcc atgtaaactg atgctggacc
      601 cccctgacct ggataattaa ggatccgaac aactttatta tacaaagttg gcattataaa
      661 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      721 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt
      781 acatggtcat agctgtttcc tggcagctct ggcc
//
