LOCUS       sequence-130367-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This vector can be used for homologous recombination and allows to 
            obtain recombinant neomycin resistant fluorescent cells that can 
            loose fluorescence after Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Gian Luca Forni
  TITLE     The plasmid is a modification of the p076 pPNT6 with the green 
            fluorescent protein gene cloned within the LoxP sites in xbaI site 
            next to Neomycin cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPNT6-eGFP
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        108..604
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             618..1421
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(681..700)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1291..1310
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1437..1898
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     enhancer        1967..2270
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2271..2474
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2425..2445
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2513..2522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2519..2521,2522..2524,2525..3235)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2519..2521,2522..2524,2525..3235)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2564..2585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2825..2844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    3352..3473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3389..3408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3443..3462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(3494..3527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3601..4100
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     5'UTR           4142..4181
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             4199..5329
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(5327..5346)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5371..5419
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(6398..6415)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6398..6414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6407..6429)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6623..6642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6742..6764
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6802..6820)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6888..6992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6993..7061,7062..7853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7211..7230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8024..8612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8513..8532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8766..8783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8900..8921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8936..8941,8942..8959,8960..8966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8974..8990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8979..9001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8998..9014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8998..9014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9035..9053
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcggccgcac gcgtcgacag cgtttaaacg aggtaccagt ggcgcgccgg cataacttcg
       61 tatagcatac attatacgaa gttataggcc tgcaggtcaa ttctaccggg taggggaggc
      121 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctggcac ttggcgctac
      181 acaagtggcc tctggcctcg cacacattcc acatccaccg gtagcgccaa ccggctccgt
      241 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
      301 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
      361 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
      421 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
      481 ggctcagggg cgggctcagg ggcggggcgg gcgcgaaggt cctcccgagg cccggcattc
      541 tcgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      601 ttcgacctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
      661 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
      721 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
      781 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
      841 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
      901 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
      961 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     1021 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1081 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1141 ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct
     1201 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1261 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1321 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1381 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aggggatcga tccgtcctgt
     1441 aagtctgcag aaattgatga tctattaaac aataaagatg tccactaaaa tggaagtttt
     1501 tcctgtcata ctttgttaag aagggtgaga acagagtacc tacattttga atggaaggat
     1561 tggagctacg ggggtggggg tggggtggga ttagataaat gcctgctctt tactgaaggc
     1621 tctttactat tgctttatga taatgtttca tagttggata tcataattta aacaagcaaa
     1681 accaaattaa gggccagctc attcctccca ctcatgatct atagatctat agatctctcg
     1741 tgggatcatt gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa
     1801 tgtgtcagtt tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat
     1861 tctcagtatt gttttgccaa gttctaattc catcagaagc tgactctaga gtcgactagt
     1921 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1981 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2041 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2101 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2161 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2221 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2281 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2341 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2401 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2461 atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat
     2521 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     2581 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     2641 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     2701 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     2761 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2821 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2881 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2941 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     3001 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     3061 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     3121 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     3181 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     3241 actcagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     3301 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     3361 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3421 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttactcgagt
     3481 ctagtagagg cctataactt cgtatagcat acattatacg aagttatgcg caggatcccc
     3541 ggttaacgcc ttaattaact cctcgagcat gctagcgtcg atttaaatgc gaattctacc
     3601 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3661 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3721 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     3781 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     3841 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     3901 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     3961 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     4021 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     4081 cctcatctcc gggcctttcg acctgcagcg acccgcttaa cagcgtcaac agcgtgccgc
     4141 agatcttggt ggcgtgaaac tcccgcacct cttcggcaag cgccttgtag aagcgcgtat
     4201 ggcttcgtac ccctgccatc aacacgcgtc tgcgttcgac caggctgcgc gttctcgcgg
     4261 ccatagcaac cgacgtacgg cgttgcgccc tcgccggcag caagaagcca cggaagtccg
     4321 cctggagcag aaaatgccca cgctactgcg ggtttatata gacggtcctc acgggatggg
     4381 gaaaaccacc accacgcaac tgctggtggc cctgggttcg cgcgacgata tcgtctacgt
     4441 acccgagccg atgacttact ggcaggtgct gggggcttcc gagacaatcg cgaacatcta
     4501 caccacacaa caccgcctcg accagggtga gatatcggcc ggggacgcgg cggtggtaat
     4561 gacaagcgcc cagataacaa tgggcatgcc ttatgccgtg accgacgccg ttctggctcc
     4621 tcatatcggg ggggaggctg ggagctcaca tgccccgccc ccggccctca ccctcatctt
     4681 cgaccgccat cccatcgccg ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag
     4741 catgaccccc caggccgtgc tggcgttcgt ggccctcatc ccgccgacct tgcccggcac
     4801 aaacatcgtg ttgggggccc ttccggagga cagacacatc gaccgcctgg ccaaacgcca
     4861 gcgccccggc gagcggcttg acctggctat gctggccgcg attcgccgcg tttacgggct
     4921 gcttgccaat acggtgcggt atctgcaggg cggcgggtcg tggcgggagg attggggaca
     4981 gctttcgggg acggccgtgc cgccccaggg tgccgagccc cagagcaacg cgggcccacg
     5041 accccatatc ggggacacgt tatttaccct gtttcgggcc cccgagttgc tggcccccaa
     5101 cggcgacctg tacaacgtgt ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc
     5161 catgcacgtc tttatcctgg attacgacca atcgcccgcc ggctgccggg acgccctgct
     5221 gcaacttacc tccgggatgg tccagaccca cgtcaccacc cccggctcca taccgacgat
     5281 ctgcgacctg gcgcgcacgt ttgcccggga gatgggggag gctaactgaa acacggaagg
     5341 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     5401 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     5461 accccaccga gaccccattg ggaccaatac gcccgcgttt cttccttttc cccaccccaa
     5521 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aagccctgcc
     5581 atagccacgg gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg
     5641 tgggggttat tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag
     5701 acccatggtt tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg
     5761 ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgc
     5821 cgccggacga actaaacctg actacggcat ctctgcccct tcttcgctgg tacgaggagc
     5881 gcttttgttt tgtattggtc accacggccg agtttccgcg ggaccccggc caggacctgc
     5941 agaaattgat gatctattaa acaataaaga tgtccactaa aatggaagtt tttcctgtca
     6001 tactttgtta agaagggtga gaacagagta cctacatttt gaatggaagg attggagcta
     6061 cgggggtggg ggtggggtgg gattagataa atgcctgctc tttactgaag gctctttact
     6121 attgctttat gataatgttt catagttgga tatcataatt taaacaagca aaaccaaatt
     6181 aagggccagc tcattcctcc cactcatgat ctatagatct atagatctct cgtgggatca
     6241 ttgtttttct cttgattccc actttgtggt tctaagtact gtggtttcca aatgtgtcag
     6301 tttcatagcc tgaagaacga gatcagcagc ctctgttcca catacacttc attctcagta
     6361 ttgttttgcc aagttctaat tccatcagaa gcttggcact ggccgtcgtt ttacaacgtc
     6421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6541 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6601 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6661 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6721 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6781 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6841 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8161 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8821 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     8881 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     8941 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9001 gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa
     9061 agctggagct ccaccgcggt g
//
