LOCUS       sequence-130395-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving APEX2-2xFYVE (2xMouse FYVE 
            domain from HRS, [marks PI3P] fused to EM peroxidase marker). Also 
            contains bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: mKate2-P2A-APEX2-2xFYVE_hrs
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..54
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    571..595
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa gttgccgtcg
       61 acttatgcct tcttgttcag ctgctcatag cagggctcac acacgcgcac ctccttctca
      121 atgccgaact tggggatggt ggagtacttg gaggagcact tgccacagaa gatctgccca
      181 catgctcggc agtgatgctt gcgggtcacc accccaaact gtactctgca ccgatggcat
      241 tcctcagcat ccacccagtc aggggctctt tcagcagcga acatggcatc actttcggat
      301 ccttgtcctt gtgccttctt gttcagctgc tcatagcagg gctcacacac gcgcacctcc
      361 ttctcaatgc cgaacttggg gatggtggag tacttggagg agcacttgcc acagaagatc
      421 tgcccacatg ctcggcagtg atgcttgcgg gtcaccaccc caaactgtac tctgcaccga
      481 tggcattcct cagcatccac ccagtcaggg gctctttcag cagcgaacat ggcatcactt
      541 tcgaattcaa ttcgaagctt gagctcgaga gccactttgt acaagaaagc tgggtcgacg
      601 gcatcagcaa acccaagctc ggaaagcttt tggtgagcct cagcgtaatc agcaaagaag
      661 gcatcttcgt ccgctgcata tttgtcaacg agagggcgga atacagggtc agacaaaaga
      721 gccttgtcag aaggtagctg aaggagacct tccttctcac cactcaacaa ctccgtgaag
      781 tatgagttgt cgaa
//
