LOCUS       sequence-130457-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 513, Emission: 
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: YPet-EB3-7
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(127..840,841..843,844..846))
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     521..540
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      843..852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccgctttac ttatagagct cgttcatgcc ctcggtgatg ccggcggcgg tcaggaactc
      181 cagcagcacc atgtggtccc gcttctcgtt ggggtccttg aacagggcgc tctggtagct
      241 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      301 gtggtcggcc agctgcacgc cgccgtcctc gatgttgtgc cggatcttga agttggcctt
      361 gatgccgttc ttctgcttgt cggcggtgat gtacacgttg tggctgttgt agttgtactc
      421 cagcttgtgg cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatccg
      481 gttcaccagg gtgtcgccct cgaacttcac ctcggcccgg gtcttgtagt tgccgtcgtc
      541 cttgaagaag atggtccgct cctgcacgta gccctcgggc atggcgctct tgaagaagtc
      601 gtgctgcttc atgtggtcgg ggtaccgggc gaagcactgc acgccgtagc ccagggtggt
      661 caccagggtg ggccagggca cgggcagctt gccggtggtg cacagcagct tcagggtcag
      721 cttgccgtag gtggcgtcgc cctcgccctc gccgctcacg ctgaacttgt ggccgttcac
      781 gtcgccgtcc agctccacca ggatgggcac cacgccggtg aacagctctt cgcctttgct
      841 caccatggtg gcgaccggtg gatccagg
//
