LOCUS       sequence-13054-e        3708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1160-HQ858704
FEATURES             Location/Qualifiers
     source          1..3708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1265..1364)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1381..1400)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1382..1400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1405..1421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1405..1421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1523..1542)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1534..2343
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1601..1620)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2490..3078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2979..2998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3232..3249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3408..3435)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3527..3613)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3662..3684
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3676..3693
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3677..3693
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgggaagca gtggagcaga tacaccgtct aaggagcagc agccacctgc tacctcaggt
      181 gctgcaatac cacctgttta tccagattgg tctagcttcc aggcatatcc cccaatccca
      241 ccacatggat tcttcccctc gcctgtggca tcaagtccac agggtcatcc tttcatgtgg
      301 ggagctcaag ctatgattcc accttatgga acaccgccac catatgtcat gtacccccct
      361 ggagtatatg cccacccatc tatgcccccg ggtgcacatc catttactcc ttatgccatt
      421 acttctccaa atggcaatgc tgatgctact ggaactactg ttgcagctgg taatactgat
      481 ggcaagccct ctgaaggcaa agataaaagt ccaacaaagc gatccaaagg aagtttgggc
      541 agcttgaaca tgcttactgg aaagaatccc agtgaacatg gtaagtcctc aggtgcatcg
      601 ggtaatggag tcacttctca aagtggcgaa agtggcagtg acagttcaag cgaagggagt
      661 gaaggaaatt ctcacaatga ttcacatcac aaggaaagtg gacaggaaca cgatggggat
      721 gttcgaagtt cccggaatgg tgtatcacgt ttgcaatctg agggaaaatt gaaccaagct
      781 atggcaatct tgcccatacc atcaagtggc ccagcaactg atccaactac aaacctaaat
      841 attgggatgg actattgggc caacacagca agctctgctc ctgcaattca tggcaaagcg
      901 acgtcaacaa cagttccagg ggctgtggtc ccagcagagc agtggacaca ggatgaacat
      961 gaactcaaaa agcaaagaag aaagcagtcc aatagggagt ctgcacgtag atctaggtta
     1021 cgtaaacagg ctgaatgtga ggaattggct caacgtgctg atgttttaaa gcaggaaaat
     1081 gcttcactta gagatgaagt aaaccgtatc agaaaagagt acgaggaact tctatcaagg
     1141 aataactcac taaaggaaaa acttgaaggc aaacaacaca aaactgatga ggcaggactt
     1201 aacaacaagc tgcaacattc tggtgatgac atccagaaaa aaggaaacaa gggtgggcgc
     1261 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1321 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1381 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1441 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1501 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1561 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1621 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1681 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1741 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1801 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1861 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1921 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1981 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2041 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2101 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2161 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2221 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2281 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2341 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2401 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2461 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2521 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2581 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2641 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2701 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2761 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2821 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2881 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2941 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3001 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3061 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3121 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3181 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3241 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3301 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3361 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3421 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3481 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3541 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3601 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3661 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
