LOCUS       sequence-130546-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  redox-sensitive red fluorescent protein rxRFP1.4, endoplasmic 
            reticulum localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Fan Y, Ai HW
  TITLE     Development of redox-sensitive red fluorescent proteins for imaging 
            redox dynamics in cellular compartments.
  JOURNAL   Anal Bioanal Chem. 2016 Jan 12.
  PUBMED    26758595
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Bioanal Chem. 2016 Jan 12."
COMMENT     Sequence Label: pER-rxRFP1.4
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             148..180
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             193..207
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttatgc tgctatccgt gcccctgctg ctcggcctgc tgggcctggc cgccgccgac
      121 ggatcccatc atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt
      181 cgggatccgt acgacgatga cgataaggat ccgagctcga gctctcctcg ttgcgctata
      241 ggtcggctga gctcacccgt ggtttccgag cggatgtacc ccgaggacgg cgccctgaag
      301 agcgagatca agaaggggct gaggctgaag gacggcggcc actacgccgc cgaggtcaag
      361 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag
      421 ttggacatcg tgtcccacaa cgaggactac accatcgtgg aacagtgcga acgcgccgag
      481 ggccgccact ccaccggcgg catggacgag ctgtacaagg gaggtacagg cgggagtctg
      541 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      601 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      661 ccctacgagg cctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc
      721 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacat taagcaccca
      781 gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaggtg ggagcgcgtg
      841 atg
//
