LOCUS       sequence-130564-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-demethylases for the degredation of caffeine and related
            methylxanthines. Can be used to measure caffeine content as 
            described in the publication listed below..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Quandt EM, Hammerling MJ, Summers RM, Otoupal PB, Slater B, Alnahhas
            RN, Dasgupta A, Bachman JL, Subramanian MV, Barrick JE
  TITLE     Decaffeination and measurement of caffeine content by addicted 
            Escherichia coli with a refactored N-demethylation operon from 
            Pseudomonas putida CBB5.
  JOURNAL   ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub
            2013 Mar 22.
  PUBMED    23654268
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4000146";
            journalName: "ACS Synth Biol"; date: "2013-06-21- 21"; volume: "2"; 
            issue: "6"; pages: "301-7"
COMMENT     Sequence Label: pDCAF3-IS5
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    9..30
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 gatttctgga attcgcggcc gcttctagag ttgacggcta gctcagtcct agggaaggtg
       61 cgaacaagtc cctgatatga gatcatgttt gtcatctgga gccatagaac agggttcatc
      121 atgagtcatc aacttacctt cgccgacagt gaattcagca gtaagcgccg tcagaccaga
      181 aaagagattt tcttgtcccg catggagcag attctgccat ggcaaaacat ggtggaagtc
      241 atcgagccgt tttaccccaa ggctggtaat ggccggcgac cttatccgct ggaaaccatg
      301 ctacgcattc actgcatgca gcattggtac aacctgagcg atggcgcgat ggaagatgct
      361 ctgtacgaaa tcgcctccat gcgtctgttt gcccggttat ccctggatag cgccttgccg
      421 gaccgcacca ccatcatgaa tttccgccac ctgctggagc agcatcaact ggcccgccaa
      481 ttgttcaaga ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac tcaaggcacc
      541 ttggtcgatg ccaccatcat tgaggcaccc agctcgacca agaacaaaga gcagcaacgc
      601 gatccggaga tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac
      661 attggtgtcg atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag
      721 catgacctca atcagctggg taatctgctg catggagagg agcaatttgt ctca
//
