LOCUS       sequence-130574-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSW_1
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..151)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(173..613)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             complement(623..664)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(683..701)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(744..763)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt ttacagtatt
       61 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
      121 tttctcgttc agcttttttg tacaaacttg tataccggtg gccgccgccc gacgatacag
      181 tcaactgtct ttgacctttg ttactactct cttccgatga tgatgtcgca cttattctat
      241 gctgtctcaa tgttagaggc atatcagtct ccactgaagc caatctatct gtgacggcat
      301 ctttattcac attatcttgt acaaataatc ctgttaacaa tgcttttata tcctgtaaag
      361 aatccatttt caaaatcatg tcaaggtctt ctcgaggaaa aatcagtaga aatagctgtt
      421 ccagtctttc tagccttgat tccacttctg tcagatgtgc cctagtcagc ggagaccttt
      481 tggttttggg agagtagcga cactcccagt tgttcttcag acacttggcg cacttcggtt
      541 tttctttgga gcacttgagc tttttaagtc ggcaaatatc gcatgcttgt tcgatagaag
      601 acagtagctt catagagccg ccggtagagt cgagtcccag aaggggatta gggattggct
      661 tccccatgat atcggtggct agcctatagt gagtcgtatt aagtactcta gccttaagag
      721 ctgtaattga actgggagtg gacacctgtg gagagaaagg caaagtggat gtcagtaaga
      781 ccaataggtg cctatcagaa a
//
