LOCUS       sequence-130619-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Matsuda T, Cepko CL
  TITLE     Controlled expression of transgenes introduced by in vivo 
            electroporation.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32. doi: 
            10.1073/pnas.0610155104
  PUBMED    17209010
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2007-01-16- 16"; 
            volume: "104"; issue: "3"; pages: "1027-32"
COMMENT     Sequence Label: pCAG-mGFP
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(80..793,794..796,797..799))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(125..146)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     474..493
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     733..754
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggcattggcc acaccagcca ccaccttctg ataggcagcc tgcacctgag gagtgcggcc
       61 gcgataattc cgggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
      121 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
      181 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      241 tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg tggcggatct
      301 tgaagttcgc cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
      361 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      421 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      481 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      541 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
      601 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
      661 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
      721 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      781 cctcgccctt gctcaccatg atcttttggt cctcatcatt cttttcaacc tgtttggttc
      841 ttctcataca gcacagcatg gtggcgaccg gtggatcatc ctcgaggaat tctttgcc
//
