LOCUS       sequence-130623-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3Xflag::tagRFP::myc in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Paix A, Folkmann A, Rasoloson D, Seydoux G
  TITLE     High Efficiency, Homology-Directed Genome Editing in Caenorhabditis 
            elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes.
  JOURNAL   Genetics. 2015 Sep;201(1):47-54. doi: 10.1534/genetics.115.179382. 
            Epub 2015 Jul 17.
  PUBMED    26187122
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.115.179382"; journalName: "Genetics"; date: 
            "2015-09"; volume: "201"; issue: "1"; pages: "47-54"
COMMENT     Sequence Label: AP575-1
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             87..794
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             795..824
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(872..888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(872..888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(885..907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    896..912
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(920..926,927..944,945..950))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    965..986
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagctcggta cccggggatc gactacaaag accatgacgg tgattataaa gatcatgata
       61 tcgattacaa ggatgacgat gacaaggtgt ctaagggcga agagctgatt aaggagaaca
      121 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaaa tgcacatccg
      181 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
      241 gccctctccc cttcgccttt gacatcctgg ctaccagctt catgtacggc agcagaacct
      301 tcatcaacca cacccagggc atccccgact tctttaagca gtcctttcct gagggcttca
      361 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
      421 gcctccagga cggctgtctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
      481 acggccctgt gatgcagaag aaaacactcg gctgggaggc caataccgag atgctgtacc
      541 ccgctgacgg cggcctggaa ggcagaagcg acatggccct gaagctcgtg ggcgggggcc
      601 acctgatctg caatttcaag accacataca gatccaagaa acccgctaag aacctcaaga
      661 tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaagaggcc gacaaagaga
      721 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
      781 ggcacaaact taatgaacaa aaactgatat cagaagagga tctggatcct ctagagtcga
      841 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1081 gtattggg
//
