LOCUS       sequence-13071-e        3405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1176-HQ858717
FEATURES             Location/Qualifiers
     source          1..3405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(962..1061)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1078..1097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1079..1097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1102..1118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1102..1118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1220..1239)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1231..2040
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1298..1317)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2187..2775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2676..2695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2929..2946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3105..3132)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3224..3310)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3359..3381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3373..3390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3374..3390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggaggcga gaatgcctcc ggggttcagg ttccacccca gggatgacga gctcgtgatg
      181 gactacctcc tacaaaagct ctccggccat ggccaccatg ccggcgctgc catcgtcgtc
      241 gacgtcgacc tcaacaagtg cgagccatgg gacctcccag attccgcgtg cgtgggtggg
      301 aaggagtggt acttcttcag cctgcgcgac cgcaagtacg cgacggggca gcgcactaac
      361 cgcgccacgc actccggcta ctggaaggcc accggcaagg accgcgccgt cgtcgccggc
      421 ggcgaggtgg cggtggggat gcgcaagacg ctggtcttct accggggccg cgcgcccagg
      481 gggaagaaga cggagtgggt catgcacgag ttccgcctcc acccccatgc cgcgccgtgc
      541 ctgctagcag cagctgcgcc aaacaaggag gactgggtgc tatgcagggt gttctacaag
      601 agcagaacaa ccagcccaag gccagaatct gaagacgccc gggacggcac cccatcagct
      661 gaaccgcaac tgccggctgc cctgccgctt gcgcccctcg ctgacaccta cgccgcccca
      721 acggttgcag agcaggtgta ctgcttctcc ggtctgccgg cactgccatt cagaagacca
      781 gtgagccttg gggaccttct ggagttcgac gcctccgaga aggaatctgt cacgacagcg
      841 atgaccagtg tctcgaacaa cactagttca gtgctggagc tggctccaaa ttgcaactgg
      901 aaccaggaaa atggcatgtc acggatgtgg agcccccttg ggataaaggg tgggcgcgcc
      961 gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     1021 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     1081 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1141 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1201 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1261 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1321 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1381 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1441 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1501 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1561 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1621 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1681 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     1741 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     1801 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     1861 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1921 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     1981 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2041 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2101 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2161 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2221 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2281 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     2341 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2401 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2461 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2521 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat
     2581 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2641 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2701 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2761 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     2821 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     2881 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     2941 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3001 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3061 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3121 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3181 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3241 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3301 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3361 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
