LOCUS       sequence-130745-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1B02
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(529..628)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(669..685)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(787..806)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(865..884)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgctac aaaaaagcag gcttcatgat ggatttgtcg ccaaacaatc
      121 agatcgagga cagaaaaccc atcctaaccg ccgacggcct ggttcagact tcgaactccc
      181 ccttcgagcc gaccatatcg caggagacgc aaacatccaa cggaatcggc ggccagtgcc
      241 atctgacggt tgaccagttg gacattgaga ttctgccgat aatctacgac attgtgcgct
      301 gcgtggaaaa ggatcctctg gagaacgccg ttaagctgcg cgagtcccag gattgcaacc
      361 acaagatctt tgaacttcaa aaacgcttcg aatcggcacg cgagcaaatc cgccagctcc
      421 ccgggatcga tttcaataag gaggagcagc aacagagact ggaactactg cgaaatcagc
      481 tgaagcttaa gcagcagcta attcgcaaat acaaggacac agagttcgac ccagctttct
      541 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      601 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
      661 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
      721 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
      781 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
      841 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc ggggcatcag
      901 gtgcgacaat ctatcgcttg tatgggaag
//
