LOCUS       sequence-130751-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_COF1C04
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(717..816)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(833..852)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(834..852)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(857..873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(975..994)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg cttcatgacc acgcgtggaa ttcggtgcaa
      121 gaagtccaca ttgacggagt tgaaaatcgc cgtgatcgga gcgcccagtg ttggcaaaag
      181 cgccctaatt gtgcgtttct tgacaaagcg ttacatcggg gagtacgatc atcagactga
      241 gaatcgctac aagcacgagg ctatggtgga tggcgaaccg gtgctctttg aaatactaga
      301 cacatgtccc aaggctgaag acgaataccc caatgcagct gagctcgttc agtgggccga
      361 tggcctattg ctggtctact cgataacaga tcgcaagagt ttcaactata tacgccgtgc
      421 caaatccgat cttcagtccg atacacccgt ccaactttgt gccaacaaag tggatatggt
      481 gcacctgcgc caggtgagcc gcgacgaggg cgaaatcctg gccaaggact ttgagtgcaa
      541 gttcagcgag gtctccgccg ccgaccacgt cgaccaggtg gccgaggttt tcaacgagct
      601 gtgcaaagag gtgctggcct ccaagcgcaa gtccaagcag agtctgctgg aaaggatgct
      661 cggtggagca cgaccctaca gccgagggaa gagcgacagc aatttgccaa aagacgaccc
      721 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      781 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
      841 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
      901 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt c
//
