LOCUS       sequence-130766-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag
            at its N-terminus and Myc tag at its C-terminus by co-transfection 
            with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pSLIK-NFLAG-hEWSR1G511A-CMyc
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(190..219)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 acgctcgctg cgcccttcgt ctgacgtggc agcgctcgcc gtgaggaggg gggcgcccgc
       61 gggaggcgcc aaaacccggc gcggaggcca gatcttgggt gggttaatta accatcttta
      121 tggctcgaga tgttatcaac cactttgtac aagaaagctg ggtctagata tctcgagtgc
      181 ggccgcctac agatcctctt ctgagatgag tttttgttcg tagggccgat ctctgcgctc
      241 ctgacggtgc tcgcctttat ccatttttcc aggtcctcca cgtcctcctc ttcttcctcc
      301 catctgttcc atcaaaggtc cagggggccc cccagggcca cctcgtcttc ctccaccaaa
      361 gccacctcgg tccatgcccc ggccaccacg gaagccacct ctgtctccac cacggccacc
      421 tctgaacatt ccaccgggac caccacgatc catgaggcca cctcttcctc cccgcatgcc
      481 accagggcca cctctgccac gatcaccacc cgggggcgga aagggtggcg ggaggaagcc
      541 ttcaggcttt ggggccttac actggttgca ctctgttctc caggcgaagt tctggtttcc
      601 acaacccgga ttgggacact gccagtctcc agctcggtgc tggacgtttc ctgctccaga
      661 ggggttcc
//
