LOCUS       sequence-130903-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_CUS1B05
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(855..954)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(971..990)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(972..990)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(995..1011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(995..1011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagc aggcttcatg ggagtaacta tccttcaacc
      121 gtatccctta cccgaaggca aatcgggtgc ccacataatc gatcagctga gcaagcgtct
      181 gctcgccctg ggcgccacac atgccggtca atttctggtg gactgcgaaa cgttcatctc
      241 gacgccacag ccgcacaatg gagcacctgg acgcgcagtc cacgtcctgc acaactccga
      301 gtatcccgcc tccacgttct cgatcatcga caatggcacc ggcaaacaag tggccattgt
      361 cgccgacaat atcttcgatc tgctcatgct caagatgacc aacaccttca cctccaaaaa
      421 gcagaccaaa atcgagtccc gtggcgctcg tttcgagtat ggtgactttg ttatcaaact
      481 cggttccgtc accatgatgg agcacttcaa gggcatcctc atcgaaatcg agtacaagtc
      541 atgcgtgatt ctggcctact gctgggaaat gatacgcgaa atgctgcagg gattcctcgg
      601 cattgctgtg aataaggact ttccctccta tttcgctcca cagacaataa tgacggcaat
      661 ggggcaacag cagctgcatg ccaagcacaa cgacatcttt gagccaatgg acaccgtgaa
      721 acaatacctg gagcagttca ccaactatag gaagcatgtg actcttatgg gcggtatggg
      781 cagtggaccg ggtagccaac aggttggtcc gaacgtgcat atgtcgccgg cggtggcggg
      841 actacatcga tccgacccag ctttcttgta caaagttggc attataagaa agcattgctt
      901 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
      961 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     1021 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaata
//
