LOCUS       sequence-131002-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses palindromic sgRNA for use in self-cloning CRISPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Arbab M, Srinivasan S, Hashimoto T, Geijsen N, Sherwood RI
  TITLE     Cloning-free CRISPR.
  JOURNAL   Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022.
  PUBMED    26527385
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 
            10.1016/j.stemcr.2015.09.022."
COMMENT     Sequence Label: sgPal1 (p2Tol-U6-sgPal1-HygR)
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    409..530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     474..493
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccgctct gtgactagtc acagaggttt aagagctatg ctggaaacag catagcaagt
      241 ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt
      301 tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat
      361 tctgcagaca aatggctcta gaggtacggc cgcttcgagc agacatgata agatacattg
      421 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      481 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
      541 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt
      601 aaaacctcta caaatgtggt aaaatcgcga aggtcgacgg tatacagaca tgataagata
      661 cattgatgag tttggacaaa ccacaactag aatgcagtga aa
//
