LOCUS       sequence-131068-       10645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generating transgenic flies for FLP-inducible DamID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Pindyurin AV, Pagie L, Kozhevnikova EN, van Arensbergen J, van 
            Steensel B
  TITLE     Inducible DamID systems for genomic mapping of chromatin proteins in
            Drosophila.
  JOURNAL   Nucleic Acids Res. 2016 Jul 8;44(12):5646-57. doi: 
            10.1093/nar/gkw176. Epub 2016 Mar 21.
  PUBMED    27001518
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw176"; journalName: "Nucleic Acids Res"; date: 
            "2016-07-8- 8"; volume: "44"; issue: "12"; pages: "5646-57"
COMMENT     Sequence Label: p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]
FEATURES             Location/Qualifiers
     source          1..10645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..40
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        46..284
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     231..252
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    304..351
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     intron          867..932
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1062..1082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1507..1641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1669..1716
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2566..2595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2717..2782
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2912..2932
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3204..3338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3367..3436
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3655..3673)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3694..3710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3694..3710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3707..3729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3718..3734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3742..3748,3749..3766,3767..3772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3787..3808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3925..3942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4096..4684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4176..4195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4855..5646,5647..5715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5478..5497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5716..5820)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5847..6302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5934..5953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6144..6165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6428..6450
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6442..6459
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6443..6459
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6466..6485
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6466..6484
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            6509..10645
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(6518..6536)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6988..7059,7461..7734,7809..8463,8525..8840,9061..9192,
                     9263..9877)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gctagcataa cttcgtataa tgtatgctat acgaagttat gagcgccgga gtataaatag
       61 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      121 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagtaaagtg
      181 caagttaaag tgaatcaatt aaaagtaacc agcaaccaag taaatcaact gcaactactg
      241 aaatctgcca agaagtaatt attgaataca agaagagaac tctgaatagg gaattgggaa
      301 ttcgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ctcggggaca
      361 ccaaatatgg cgatctcggc cttttcgttt cttggagctg ggacatgttt gccatcgatc
      421 catctaccac cagaacggcc gttagatctg ctgccaccgt tgtttccacc gaagaaacca
      481 ccgttgccgt aaccaccacg acggttgttg ctaaagaagc tgccaccgcc acggccaccg
      541 ttgtagccgc cgttgttgtt attgtagttg ctactgttat ttctggcact tcttggtttt
      601 cctcttaagt gaggaggaac ataaccattc tcgttgttgt cgttgatgct taaattttgc
      661 acttgttcgc tcagttcagc cataatatga aatgcttttc ttgttgttct tacggaatac
      721 cacttgccac ctatcaccac aactaacttt ttcccgttcc tccatctctt ttatattttt
      781 tttctcgagg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
      841 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
      901 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      961 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     1021 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     1081 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     1141 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     1201 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     1261 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     1321 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     1381 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     1441 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     1501 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1561 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1621 gtatcttatc atgtctggat ctgacatggt aagtaagctt gggctgcaga agttcctatt
     1681 ccgaagttcc tattctctag aaagtatagg aacttcgaat tcaacatgaa gaaaaatcgc
     1741 gcttttttga agtgggcagg gggcaagtat cccctgcttg atgatattaa acggcatttg
     1801 cccaagggcg aatgtctggt tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc
     1861 gacttttctc gttatatcct tgccgatatc aatagcgacc tgatcagtct ctataacatt
     1921 gtgaagatgc gtactgatga gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca
     1981 aattgcgccg aggtttacta tcagttccgc gaagagttca acaaaagcca ggatccgttc
     2041 cgtcgggcgg tactgttttt atatttgaac cgctacggtt acaacggcct gtgtcgttac
     2101 aatctgcgcg gtgagtttaa cgtgccgttc ggccgctaca aaaaacccta tttcccggaa
     2161 gcagagttgt atcacttcgc tgaaaaagcg cagaatgcct ttttctattg tgagtcttac
     2221 gccgatagca tggcgcgcgc agatgatgca tccgtcgtct attgcgatcc gccttatgca
     2281 ccgctgtctg cgaccgccaa ctttacggcg tatcacacaa acagttttac gcttgaacaa
     2341 caagcgcatc tggcggagat cgccgaaggt ctggttgagc gccatattcc agtgctgatc
     2401 tccaatcacg atacgatgtt aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc
     2461 aaagttcgac gcagtataag cagcaacggc ggcacacgta aaaaggtgga cgaactgctg
     2521 gctttgtaca aaccaggagt cgtttcaccc gcgaaaaaag ccggtgaaca gaaactcatc
     2581 tctgaagagg atctgggcgc gccatttaaa tggccggccg cgatcgcggc cgctctagag
     2641 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     2701 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     2761 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     2821 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     2881 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     2941 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     3001 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     3061 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     3121 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3181 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3301 tgtccaaact catcaatgta tcttatcatg tctggatcca ctagtgtcga cgatgtaggt
     3361 cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta
     3421 ctccacctca cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc
     3481 ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac
     3541 ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg
     3601 gttctcgacg gtcacggcgg gcatgtcgac actagttcta gccagctttt gttcccttta
     3661 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3721 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3781 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3841 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3901 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4741 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4801 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4861 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4921 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4981 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5041 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5101 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5161 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5221 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5281 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5341 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5401 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5461 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5521 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5581 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5641 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5701 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5761 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5821 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     5881 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5941 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6001 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6061 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6121 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6181 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6241 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     6301 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     6361 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     6421 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
     6481 tagggcgaat tgggtacgta ccgggcccct agtatgtatg taagttaata aaacccattt
     6541 ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata tcattgaact
     6601 tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca ggttctttcg
     6661 attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc tccttctctg
     6721 tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc gccaccgttt
     6781 gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg tttcgtgacg
     6841 aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa actcctctcg
     6901 cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg ggtgggtaag
     6961 caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct attaattcgc
     7021 ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac atgtgcatac
     7081 atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt ctgcggcgtg
     7141 agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt gacacgaaac
     7201 agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat atcatgatca
     7261 agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata taacaaccag
     7321 atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc tacttcgtag
     7381 gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta gttgatattt
     7441 gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc attaaccagg
     7501 gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg gaggactccg
     7561 gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat atggacatct
     7621 ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg acacgcggac
     7681 tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag aacggtgagt
     7741 ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat taccaatttg
     7801 aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg gcagttccgg
     7861 tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg gcatccaagt
     7921 atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg agatgcaggc
     7981 caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg ccagggaaca
     8041 cctgattttc caggccatgg tgcggatgcc acgacatctg acctatcggc agcgagtggc
     8101 ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca cgatcatcgg
     8161 tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg cattcgcctc
     8221 cgaggcacta accgatccgc cgcttctgat ctgcgatgag cccacctccg gactggactc
     8281 atttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg gcaagaccgt
     8341 catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca agatccttct
     8401 gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg acttcttttc
     8461 ctagtgagtt cgatgtgttt attaagggta tctagcatta cattacatct caactcctat
     8521 ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac gtacaggtgt
     8581 tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag atatgcgaca
     8641 attttgctat tagcaaagta gcccgggata tggagcagtt gttggccacc aaaaatttgg
     8701 agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg ttcatgcagt
     8761 tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc ctcgtaaaag
     8821 tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat aacgcttaca
     8881 attcttggaa acaaattcgc tagattttag ttagaattgc ctgattccac acccttctta
     8941 gtttttttca atgagatgta tagtttatag ttttgcagaa aataaataaa tttcatttaa
     9001 ctcgcgaaca tgttgaagat atgaatatta atgagatgcg agtaacattt taatttgcag
     9061 atggttgcca tcttgattgg cctcatcttt ttgggccaac aactcacgca agtgggcgtg
     9121 atgaatatca acggagccat cttcctcttc ctgaccaaca tgacctttca aaacgtcttt
     9181 gccacgataa atgtaagtct tgtttagaat acatttgcat attaataatt tactaacttt
     9241 ctaatgaatc gattcgattt aggtgttcac ctcagagctg ccagttttta tgagggaggc
     9301 ccgaagtcga ctttatcgct gtgacacata ctttctgggc aaaacgattg ccgaattacc
     9361 gctttttctc acagtgccac tggtcttcac ggcgattgcc tatccgatga tcggactgcg
     9421 ggccggagtg ctgcacttct tcaactgcct ggcgctggtc actctggtgg ccaatgtgtc
     9481 aacgtccttc ggatatctaa tatcctgcgc cagctcctcg acctcgatgg cgctgtctgt
     9541 gggtccgccg gttatcatac cattcctgct ctttggcggc ttcttcttga actcgggctc
     9601 ggtgccagta tacctcaaat ggttgtcgta cctctcatgg ttccgttacg ccaacgaggg
     9661 tctgctgatt aaccaatggg cggacgtgga gccgggcgaa attagctgca catcgtcgaa
     9721 caccacgtgc cccagttcgg gcaaggtcat cctggagacg cttaacttct ccgccgccga
     9781 tctgccgctg gactacgtgg gtctggccat tctcatcgtg agcttccggg tgctcgcata
     9841 tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc gaaataactg
     9901 cttgtttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     9961 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
    10021 tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa aagacttaca
    10081 cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg ggatatattt
    10141 tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca ccacaccacg
    10201 tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc accttttgtt
    10261 cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggttcgctca aatggttccg
    10321 agtggttcat ttcgtctcaa tagaaattag taataaatat ttgtatgtac aatttatttg
    10381 ctccaatata tttgtatata tttccctcac agctatattt attctaattt aatattatga
    10441 ctttttaagg taattttttg tgacctgttc ggagtgatta gcgttacaat ttgaactgaa
    10501 agtgacatcc agtgtttgtt ccttgtgtag atgcatctca aaaaaatggt gggcataata
    10561 gtgttgttta tatatatcaa aaataacaac tataataata agaatacatt taatttagaa
    10621 aatgcttgga tttcactgga actag
//
