LOCUS       sequence-13112-e        4998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1498-HQ858756
FEATURES             Location/Qualifiers
     source          1..4998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2555..2654)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2671..2690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2672..2690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2695..2711)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2695..2711)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2813..2832)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2824..3633
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2891..2910)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3780..4368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4269..4288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4522..4539
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4698..4725)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4817..4903)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4952..4974
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4966..4983
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4967..4983
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catggcgcag agcaactggg aagcggataa gatgctcgac
      181 gtgtatatct atgactacct gctgaagagg aacctgcaga cgacggccaa ggctttcatg
      241 accgagggaa aggttgctgc tgatccagtt gcaattgatg ctcctggagg gtttctgttt
      301 gagtggtggt ctgtcttttg ggatatattc atcgcaagga caaatgaaaa gcattctgaa
      361 gttgcagcgg catatctaga ggcacagcaa ataaaagcaa gagagcacca gcagcagatg
      421 cagatgcagc aattgcaact tatgcatcaa agacatgctc aacttcagcg aactaacgcg
      481 aatcatcctt cacttaatgg tccaataaat gctttaaatt ctgagggcat tttggggcca
      541 tcaacagcta gtgtcttagc tgccaagatg tatgaagagc gattgaagca cccacattca
      601 atggagtctg agggatcaca acttattgaa gctagtagaa tggctcttct caagtcagca
      661 acaaaccatg cagggcaatt agttccaggg actcctggaa atgtgtctac aactctgcag
      721 caaatccagg cacggaatca gcaaacgatt gatattaaga gtgaaggtaa catgggagta
      781 ccccagagat ctttacccat ggatccatca tctttatacg gtcaaggatt aattcaacct
      841 aaacctggat tgagcggtgc agggctgaac caaggtgtaa gcggtttacc actgaagggc
      901 tggccattaa ctggaattga ccagttaagg ccgaatttgg gtgctcaaat gcagaagcca
      961 tttctttcaa cacagtcaca gttccaactt atgtccccac agcagcaaca acaatactta
     1021 gcacaggccc aagcacaagg gaaccttgga aactcaacta attatgggga tattgatccc
     1081 cgcagactta cagcattgac caggggtagt ttgaatggta aagatagtca gcctgcagga
     1141 acggatggtt gtattagttc ccctatgcaa tccagttcac ccaaagtcag accagaccaa
     1201 gagtatctca tgaagatgca gcagacatct tcccagcaac cccaggaaca gcttcagcag
     1261 cagcagcaga accaacagca gcaacagagc caacaacaaa tgcaacagaa caatagaaaa
     1321 agaaagcagc ctacttcttc aggaccagca aatagtaccg gtacaggaaa cactgtgggt
     1381 cctgcaaact ccccaccatc tacaccatcc acacatactc ctggggatgg acttggaatg
     1441 ggtggtaata tgcgccacgt tccaaagaac ttaatgattt atggtgcgga tggaactgga
     1501 cttgcctcct cttcaaatca aatggatgac ctggagcagt ttggtgatgt gggctctttg
     1561 gacgataatg ttgaatcctt cttatccaat gatgatggag atcccaggga tatttttgct
     1621 gcactcaaaa gaagtcctgc agagcctagc ccagccgttt caaaaggttt tactttcagt
     1681 gaagtcaact gttggcgcac aagcaacagc aaaattgttt gctgccactt ctcttcagat
     1741 ggcaagattt tggctagtgc aggacatgaa aagaaggctg tactttggaa catggaaaat
     1801 tttcagacac agtatacacc agaagagcat gcccttatta tcactgatgt ccgtttcagg
     1861 cctaactcta gtcagctggc aacatcttct tttgatagaa ctattaaatt atggaatgct
     1921 gccgatcctg gattctcttt gcatacattc actgggcaca acggccaggt cacatcatta
     1981 gattttcatc caaagaaaac agaccttcta tgctcttgtg atggcagtgg tgaaatccgg
     2041 tactggaatg tgacccagtt tgcatgcttg cgtgccataa agggtggtag tgcccaagtc
     2101 cggtttcagc ctcatgttgg acagttttta gccgcagctg ctgaaaacgt ggtttccata
     2161 tttgacattg agacacatgg gaaaaaatac actctacagg gccataacac tgatgtgcag
     2221 tcagtgtgct gggacaacaa tggggaatat attgcatctg tcagtcagga cctagttaag
     2281 gtttggtcaa tgtcaactgg ggattgcatt tatgagctta gttccaatgg aaacaagttc
     2341 cattcttgcg tgttccaccc gagctattcc aatctcttgg ttattggagg ctaccagtct
     2401 ctcgaggttt ggaacatggt gaaaaaccag agcctaaccg tacaagctca tgaaggtcta
     2461 attgcggcgc tggcgcagtc accagtcaca gggatgatag cttctgctag ccatgacaac
     2521 tctgtcaagg tgtggaaaaa gggtgggcgc gccgacccag ctttcttgta caaagttggc
     2581 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2641 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2701 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2761 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2821 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2881 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2941 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     3001 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     3061 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     3121 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3181 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3241 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3301 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3361 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3421 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3481 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3541 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3601 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3661 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3721 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3781 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3841 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3901 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3961 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4021 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4081 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4141 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     4201 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4261 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4321 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4381 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4441 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4501 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4561 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4621 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4681 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4741 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4801 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4861 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4921 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     4981 agtcttaagc tcgggccc
//
