LOCUS       sequence-13116-e        4494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1502-HQ858760
FEATURES             Location/Qualifiers
     source          1..4494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             112..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(2051..2150)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2167..2186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2168..2186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2191..2207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2191..2207)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2309..2328)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2320..3129
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2387..2406)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3276..3864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3765..3784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4018..4035
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4194..4221)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4313..4399)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4448..4470
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4462..4479
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4463..4479
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc cttgtttaac
      121 tttaagaagg agcccttcac catgaccgtc gacgagctga agctgcaggc gagggccagc
      181 ggcggccacg gcgccaagga ccagttcccc gtcggcatgc gcgtgctcgc cgtcgacgac
      241 gacccgacct gcctcaagat cctcgagaac ctcttgctcc gctgccagta ccatgtgaca
      301 acaacgggac aggcagccac ggccttgaag ctgctgaggg agaagaagga ccagtttgac
      361 cttgtgatca gcgacgtcca catgccggac atggacggct tcaagctcct cgagcttgtg
      421 ggcctcgaga tggacctccc ggtcattatg ctgtctgcaa atggggagac acagacagtc
      481 atgaagggga ttacccatgg agcatgtgac tacctgctga agccagtgcg tattgagcag
      541 ctgaggacta tatggcagca tgtggttagg cggaggagtt gtgacgccaa gaacagtggc
      601 aatgacaacg atgattcggg taaaaagttg caggtggtga gcgctgaagg tgacaatggc
      661 ggtgttaatc gcaacaagag aatttcacgc aagggtaggg atgataatgg tgatgacggt
      721 gatgattctg atgacaacag taatgagaat ggagactcgt cgagccagaa gaagccaagg
      781 gttgtgtggt ctgttgaatt gcaccgaaag tttgtggctg ctgtcaacca gcttggcatt
      841 gacaaggctg ttccaaagaa aatattggat cttatgaatg ttgagaacat cacaagggag
      901 aacgttgcaa gtcatctgca gaagtatcgg ctgtatctga aaagactcag cgcagatgca
      961 agcaggcagg ctaacctaac tgctgcattt ggaggaagaa accctgctta tgtgaacatg
     1021 ggactagacg ccttcaggca gtacaatgca tatgggagat accggccagt tccaaccact
     1081 aaccattcac agccaaacaa cctccttgca aggatgaact ctcctgcatt tggtatgcat
     1141 ggtctgctgc cttcgcagcc acttcagatt ggacacaacc agaacaatct gagcacttcc
     1201 ctagggaatg tcggggggat gaacaacggc aacctgatca ggggtgcgca tatgccactg
     1261 caggatacta gcaaatgctt tcctactggc ccttctggta attcgttcgc gaacatatca
     1321 aacagcacac aactggttac gacaaacaac ttgccattac agtctcttga gccaagcaac
     1381 caacaacacc ttggtcggct gcattcctct gcagaccctt tcaactcatt tgttggtgag
     1441 cccccccagt ttgcagatct tggaaggtgc aacaccacct ggccaaccgc ggtctcatca
     1501 tccaatgttc aggagattgg ccagaaggac aggatcgtga ataggcccaa gcttgagcct
     1561 ctctcaagct ttacagaagc atccagtcag atccctttgc tgggaagtga aatgcagagc
     1621 caccaagtag catcactagc cagcaacggt cttccgatgc cgttcactca ggaagcggtg
     1681 cccttcgctt atggaagcag cacgaactcg agagagatgc tgaataacaa ccttgcgctt
     1741 agtaattcag gcgtcaactc tacattgccg aaccttcgca tagacggttc tgttgttcca
     1801 gggcagacac tgggtggtag caattcaggt ggttgtgttg tcccccctct gcaagatggc
     1861 aggattgatc accaagctgt cagcagtcat ctaaactaca acaacgaact catggggaca
     1921 ggtaggctgc aaaggggact tagtggtggt ctggatgaca ttgtcgtgga catgtttagg
     1981 ccggaccgcg cagacgatgg cgtctccttc attgatgggg actgggaact ggtcaagggt
     2041 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2101 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     2161 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     2221 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     2281 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     2341 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     2401 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     2461 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     2521 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     2581 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2641 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2701 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2761 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2821 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2881 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2941 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     3001 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     3061 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     3121 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     3181 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     3241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3421 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3661 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3901 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3961 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4021 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4141 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     4201 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     4261 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     4321 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     4381 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     4441 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
