LOCUS       sequence-131202-        8156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3C protein (truncated at cleavage site P1; ie, 
            short), C-terminally fused to alkaline phosphatase + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3c(S)-AP-His
FEATURES             Location/Qualifiers
     source          1..8156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4736..4753
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4779..4900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4816..4835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4870..4889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4938..4955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5109..5697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5189..5208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5868..6659,6660..6728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6491..6510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6729..6833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6901..6919
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6957..6979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6981..7001)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6986..7316
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7167..7302
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7229..7248
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7453..7472
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7539..7994)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7676..7697)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7888..7907
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8120..8142
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8134..8151
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8135..8151
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcattccg
     1621 ggcgatttgt gtgttggttg gagtatttat ttgttccatt tgtgtacgag gatcttccca
     1681 gccccaagca agagtttatt taacatttga tgagcttcga gaaaccaaaa cctctgagta
     1741 ctttagtctg tcccaccagc agttagacta cagaatattg ctgatggatg aagatcaaga
     1801 ccggatatat gtggggagca aagaccacat cctgtctttg aatatcaaca atatcagtca
     1861 agaacctttg agtgttttct ggccagcatc aacaatcaaa gttgaagagt gcaaaatggc
     1921 tggcaaagat cctacacatg gctgtggaaa tttcgtccgg gtgattcaga cattcaaccg
     1981 tactcacctg tatgtctgtg ggagtggagc gttcagccca gtgtgcacct acctgaaccg
     2041 gggaaggagg tcagaggacc aggtattcat gatcgactct aagtgtgaat ctggcaaagg
     2101 acgatgctct ttcaacccga atgtgaacac tgtgtctgtt atgatcaatg aggaactctt
     2161 ctcaggaatg tatatagact tcatgggaac agatgctgct attttccgaa gtttaactaa
     2221 gaggaatgca gttcgaactg atcaacataa ttcaaaatgg ctgagtgaac ccatgtttgt
     2281 ggacgcacat gtgatcccag atggcactga tccaaatgat gctaaggtct atttcttctt
     2341 caaagaaaga ctgactgaca acaataggag cacaaaacag attcattcca tgattgcaag
     2401 aatatgccct aatgacactg gtggacaacg tagtcttgtc aacaagtgga ccacattctt
     2461 aaaggcaaga cttgtatgct cagtcacaga tgaagatggc ccagagacac attttgatga
     2521 actagaggat gtctttctgc tggaaactga caatccaagg acaacactcg tgtatggcat
     2581 cttcaccaca tcaagctctg tttttaaggg atcggcagtg tgtgtgtatc atttatctga
     2641 tatacagact gtattcaatg ggccctttgc ccacaaggaa gggcccaatc accagctgat
     2701 ctcctatcaa ggtagaatcc catatcctcg cccaggaact tgcccaggag gggcctttac
     2761 acccaatatg agaaccacca aggacttccc agatgacgtt gtcactttta ttcggaacca
     2821 ccctctcatg tacaattcca tctaccccat ccacagaagg cctctgatag tccgcatagg
     2881 cactgactac aagtacacaa agattgctgt ggaccgtgtc aacgctgctg atgggagata
     2941 ccacgttctg tttctgggca cagatcgggg caccgtgcag aaggtcgtag tccttcctac
     3001 caatagctct gccagtgggg aactcatcct ggaggagctg gaagtcttca agaatcatgt
     3061 tcccataaca acaatgaaaa tctcatccaa aaagcaacag ttgtacgtga gctccaatga
     3121 gggggtttcc caagtctctc tgcatcgctg ccatatctac ggcacagcct gtgcggactg
     3181 ctgcttggcg agggatccat actgtgcctg ggatggccac tcttgctcta ggttctaccc
     3241 cactgggggc gcgcctggat cttccggaat catcccagtt gaggaggaga acccggactt
     3301 ctggaaccgc gaggcagccg aggccctggg tgccgccaag aagctgcagc ctgcacagac
     3361 agccgccaag aacctcatca tcttcctggg cgatgggatg ggggtgtcta cggtgacagc
     3421 tgccaggatc ctaaaagggc agaagaagga caaactgggg cctgagatac ccctggccat
     3481 ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat gtagacaaac atgtgccaga
     3541 cagtggagcc acagccacgg cctacctgtg cggggtcaag ggcaacttcc agaccattgg
     3601 cttgagtgca gccgcccgct ttaaccagtg caacacgaca cgcggcaacg aggtcatctc
     3661 cgtgatgaat cgggccaaga aagcagggaa gtcagtggga gtggtaacca ccacacgagt
     3721 gcagcacgcc tcgccagccg gcacctacgc ccacacggtg aaccgcaact ggtactcgga
     3781 cgccgacgtg cctgcctcgg cccgccagga ggggtgccag gacatcgcta cgcagctcat
     3841 ctccaacatg gacattgacg tgatcctagg tgggggccga aagtacatgt ttcgcatggg
     3901 aaccccagac cctgagtacc cagatgacta cagccaaggt gggaccaggc tggacgggaa
     3961 gaatctggtg caggaatggc tggcgaagcg ccagggtgcc cggtacgtgt ggaaccgcac
     4021 tgagctcatg caggcttccc tggacccgtc tgtggcccat ctcatgggtc tctttgagcc
     4081 tggagacatg aaatacgaga tccaccgaga ctccacactg gacccctccc tgatggagat
     4141 gacagaggct gccctgcgcc tgctgagcag gaacccccgc ggcttcttcc tcttcgtgga
     4201 gggtggtcgc atcgaccatg gtcatcatga aagcagggct taccgggcac tgactgagac
     4261 gatcatgttc gacgacgcca ttgagagggc gggccagctc accagcgagg aggacacgct
     4321 gagcctcgtc actgccgacc actcccacgt cttctccttc ggaggctacc ccctgcgagg
     4381 gagctccatc ttcgggctgg cccctggcaa ggcccgggac aggaaggcct acacggtcct
     4441 cctatacgga aacggtccag gctatgtgct caaggacggc gcccggccgg atgttaccga
     4501 gagcgagagc gggagccccg agtatcggca gcagtcagca gtgcccctgg acgaagagac
     4561 ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg caggcgcacc tggttcacgg
     4621 cgtgcaggag cagaccttca tagcgcacgt catggccttc gccgcctgcc tggagcccta
     4681 caccgcctgc gacctggcgc cccccgccgg caccaccgac gccgcgcacc cgggtcatca
     4741 tcaccatcac cattaatcta gagatcttat taaagcagaa cttgtttatt gcagcttata
     4801 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4861 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgactctag
     4921 ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6841 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     6901 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     6961 tgacacatgc agctcccgga ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7021 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     7081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7141 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7321 tcccgggagc ttgtatatcc attttcgtcc cggagacggt cacagcttgt ctgtaagcgg
     7381 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     7441 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     7501 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     7561 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     7621 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     7681 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     7741 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     7801 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     7861 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     7921 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     7981 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8041 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8101 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatt
//
