LOCUS       sequence-131273-        7859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the NCAM1, isoform 1.0.1 
            protein, C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Ncam1_1.0.1-Fc-His
FEATURES             Location/Qualifiers
     source          1..7859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1650..1661
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4439..4456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4482..4603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4519..4538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4573..4592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4641..4658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4812..5400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4892..4911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5571..6362,6363..6431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6194..6213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6432..6536)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6604..6622
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6660..6682)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6684..6704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6689..7019
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6870..7005
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6932..6951
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7156..7175
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7242..7697)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7379..7400)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7591..7610
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7823..7845
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7837..7854
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7838..7854
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgctgcgaac
     1621 taaggatctc atctggactt tgtttttcct gggaactgca gtttccctgc aggtagatat
     1681 tgttcccagc caaggagaaa tcagcgttgg agagtccaaa ttcttcctgt gtcaagtggc
     1741 aggagatgcc aaagataagg acatctcctg gttctccccc aatggggaga agctgagccc
     1801 aaaccagcag cggatctcag tggtgtggaa tgatgacgac tcctctaccc tcaccatcta
     1861 caacgccaac atcgacgatg ccggcatata caagtgtgtg gtcacggctg aggacggcac
     1921 ccagtctgag gccactgtca acgtgaagat cttccagaag ctcatgttca agaatgcacc
     1981 aaccccacag gagtttaagg aaggggagga tgctgtgatt gtctgtgatg tggtcagctc
     2041 cctgcctcca accatcatct ggaaacacaa aggccgagat gtcattctga aaaaagacgt
     2101 ccggttcata gtcctgtcca acaactacct gcagatcagg ggcatcaaga aaacagatga
     2161 gggtacttac cgctgtgagg gcaggatcct ggcccgcggg gaaatcaact tcaaggacat
     2221 tcaggtcatt gtgaatgtac cacccactgt ccaggccaga cagagcatcg tgaatgccac
     2281 tgccaacctg ggccagtctg tcaccctggt gtgtgatgcc gatggcttcc cagagcccac
     2341 catgagctgg acaaaggatg gggaacccat agagaacgag gaggaagatg acgagaagca
     2401 catcttcagt gatgacagct ccgagctgac catcaggaat gtggataaaa acgacgaggc
     2461 cgaatacgtc tgcatcgcag agaacaaggc tggcgagcag gatgcctcca tccacctcaa
     2521 ggtctttgca aagcccaaaa tcacctatgt agagaatcag acggccatgg aactagagga
     2581 gcaagtcact ctgacctgtg aagcctccgg agaccccatc ccctccatca cctggagaac
     2641 atccacccgg aacatcagca gtgaagaaaa ggcatcgtgg actcgaccag agaagcaaga
     2701 gactctggat gggcacatgg tggttcgcag ccatgctcgt gtgtcctcct tgaccctgaa
     2761 gagcatccag tacacagatg ctggagaata catctgcact gccagcaaca ccatcggcca
     2821 ggattcccag tccatgtacc tcgaagttca atatgctccc aagctccagg gccctgtggc
     2881 tgtgtacact tgggaaggga accaggtgaa catcacctgt gaggtcttcg cctacccaag
     2941 tgccacaatc tcctggttcc gagatggtca gttgctgcca agctccaact acagcaatat
     3001 caagatctac aacaccccat ctgctagcta tctggaggtg acccctgatt cagaaaatga
     3061 ctttggaaac tacaactgta cagcagtgaa ccgtattgga caggagtcct tggaattcat
     3121 ccttgttcaa gcagacacac cgtcttctcc atccattgac cgggtggaac catactccag
     3181 cacagcgcag gtgcagtttg atgagccaga ggccacaggg ggcgtgccca ttctcaagta
     3241 caaggctgag tggaagtcgc tgggcgaaga atcctggcat ttcaagtggt atgatgccaa
     3301 agaagccaac atggaaggca ttgtcaccat catgggcctg aaacctgaga cgaggtactc
     3361 ggtacgactg gcggccctca acggcaaggg gctgggcgag atcagtgcag ccactgagtt
     3421 caagacacag ccagtccggg aacccagtgc acccaagctg gaagggcaga tgggagagga
     3481 cgggaactcc atcaaggtga acctgatcaa gcaggatgac ggaggctccc ccatcagaca
     3541 ctatctggtc aagtacagag cgaagctcgc ctctgagtgg aaaccggaaa tcaggctccc
     3601 atccggcagt gaccacgtca tgctcaagtc cctggactgg aacgcagagt atgaagtcta
     3661 tgtggtagct gaaaaccagc aaggaaaatc caaggcagct cactttgtgt tcaggacctc
     3721 agcccagccc acggccatcc cagccactag tgacaaaact cacacatgcc caccgtgccc
     3781 agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac
     3841 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     3901 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     3961 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     4021 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     4081 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtatac
     4141 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa
     4201 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     4261 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct
     4321 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     4381 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aaggcggcca
     4441 tcatcaccat caccattaat ctagagatct tattaaagca gaacttgttt attgcagctt
     4501 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4561 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggtcgactc
     4621 tagctagact cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4981 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5041 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5101 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5161 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5221 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5281 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5341 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5401 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5461 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5521 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5581 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5641 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5701 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5761 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5821 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5881 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5941 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6001 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6061 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6121 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6181 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6241 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6301 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6361 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6421 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6481 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6541 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     6601 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     6661 ctctgacaca tgcagctccc ggaggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6721 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7021 agctcccggg agcttgtata tccattttcg tcccggagac ggtcacagct tgtctgtaag
     7081 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7141 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7201 aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat
     7261 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     7321 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     7381 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     7441 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     7501 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     7561 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     7621 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     7681 gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7741 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7801 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatt
//
